| 41. | The dye eosinate of methylene blue belongs to which group? |
| a. | Acidic dye |
| b. | Basic dye |
| c. | Neutral dye |
| d. | Oxazine dye |
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Answer: (c).Neutral dye
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| 42. | What is the correct order of staining reagents in Gram-Staining? |
| a. | Crystal violet, alcohol, iodine solution, safranin |
| b. | Crystal violet, iodine solution, alcohol, safranin |
| c. | Crystal violet, safranin, alcohol, iodine solution |
| d. | Iodine solution, crystal violet, alcohol, safranin |
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Answer: (b).Crystal violet, iodine solution, alcohol, safranin
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| 43. | Which bacteria appears purple-violet colour after staining? |
| a. | Gram-positive |
| b. | Gram-negative |
| c. | Both Gram-positive and Gram-negative |
| d. | Neither Gram-positive nor Gram-negative |
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Answer: (a).Gram-positive
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| 44. | Which of the following are true for Gram-negative bacteria? |
| a. | upon alcohol treatment, the permeability of the cell wall increases |
| b. | crystal violet-iodine (CV-I) complex is extracted |
| c. | pore size decreases and the CV-I complex cannot be extracted |
| d. | alcohol treatment increases the permeability of the cell wall and the CV-I complex can be extracted |
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Answer: (d).alcohol treatment increases the permeability of the cell wall and the CV-I complex can be extracted
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| 45. | Gram-positive bacteria are usually more susceptible to? |
| a. | streptomycin |
| b. | tetracyclin |
| c. | penicillin |
| d. | ampicillin |
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Answer: (c).penicillin
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