Q. The enzyme which specifically catalyzes β (1 → 4) glycosidic linkages of NAM (N-acetyl muramic acid) and NAG (N-acetyl glucosamine) present in bacterial peptidoglycan is _______________

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Q. ___________ is a divalent chelator which can be used to extract enzymes from different sources.

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Q. Which one of the following is not an example for ionic-detergent?

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Q. Pick odd one out.

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Q. The phenomenon wherein the osmotic pressure created due to presence of solutes inside and outside the cells will be equal is referred to as ________

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Q. Which chemical is used for extracting enzymes by osmotic shock treatment?

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Q. Which of the following is not a physical method for extraction of enzymes?

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Q. ____________ is a phenomenon of assuming frequencies more than 20 kHz which is above the human audibility.

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Q. The procedure in which cell lysis occur because of shock waves which are formed due to collapses occurring in buffered solution containing cells is referred to as _______________

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Q. Homogenization cannot be done by using which one of the following?

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Q. Homogenization utilizing ____________ is one where in manual pressure is created for cell lysis.

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Q. A _________ has long teflon tube about 30-40 cm long which is bulged at the end and has an automated machine for providing the pressure.

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Q. Homogenization performed by passing the cells at high pressure through narrow orifice which can also cause cell rupture is referred to as _______________

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Q. The technique in which the cells are subjected to cold temperature of about -15° C to -25° C is referred to as _____________

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Q. ____________ technique is nothing but a cold or psychrophilic shock.

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Q. Freezing technique is not preferred unless otherwise there is a need.

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Q. Mortar and pestle, french press and homogenizer are widely used to lyse the cells by ultrasonication.

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Q. __________ is a method which is highly used for lab scale procedures than large scale work.

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Q. _________________ is the main contaminant present in the resultant free extract from cells.

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Q. Enzymes can be isolated from cell free extract by which one of the following methods?

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