Q. Which chemical is used for extracting enzymes by osmotic shock treatment?

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Q. Which of the following is not a physical method for extraction of enzymes?

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Q. ____________ is a phenomenon of assuming frequencies more than 20 kHz which is above the human audibility.

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Q. The procedure in which cell lysis occur because of shock waves which are formed due to collapses occurring in buffered solution containing cells is referred to as _______________

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Q. Homogenization cannot be done by using which one of the following?

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Q. Homogenization utilizing ____________ is one where in manual pressure is created for cell lysis.

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Q. A _________ has long teflon tube about 30-40 cm long which is bulged at the end and has an automated machine for providing the pressure.

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Q. Homogenization performed by passing the cells at high pressure through narrow orifice which can also cause cell rupture is referred to as _______________

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Q. The technique in which the cells are subjected to cold temperature of about -15° C to -25° C is referred to as _____________

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Q. ____________ technique is nothing but a cold or psychrophilic shock.

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Q. Freezing technique is not preferred unless otherwise there is a need.

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Q. Mortar and pestle, french press and homogenizer are widely used to lyse the cells by ultrasonication.

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Q. __________ is a method which is highly used for lab scale procedures than large scale work.

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Q. _________________ is the main contaminant present in the resultant free extract from cells.

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Q. Enzymes can be isolated from cell free extract by which one of the following methods?

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Q. Which of the following methods cannot be used to isolate enzymes from cell free extract?

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Q. Which one if the chemicals is not used to isolate enzymes from cell free extract?

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Q. ___________ is responsible for maintaining biologically native structure of an enzyme and even nucleic acid.

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Q. Increase or decrease in _________ leads to protonation or deprotonation of bases which leads to melting of DNA in turn isolating the enzyme after centrifugation.

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Q. The increase in pH affects the inter H₂ bonds and intramolecular hydrophobic bonds are majorly affected leading to denaturation of DNA.

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