Q. What does the following equation represents?

ϕ=\(\frac{kPA_f}{ηD_f}\)

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Q. The presence of enzyme in the sample can be detected by _________________

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Q. The method which relies on differences in partitioning behavior between a flowing mobile phase and a stationary phase to separate the components in a mixture is referred to as _________________

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Q. Which chromatography technique consist of a stationary phase immobilized on a glass plate and an organic solvent acting as a mobile phase?

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Q. If the solvent phase in TLC was 80:20 mixture of hexane and ethyl acetate, the polar compounds will not move as much as non-polar compounds.

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Q. When the solvent phase in TLC is 20:80 mixture of hexane and ethyl acetate, the polar compounds will not move as much as non-polar compounds.

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Q. Pick odd one out.

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Q. What is the coloring agent used to detect laccase?

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Q. On spraying the coloring agent, the enzyme is detected as a __________

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Q. The ratio of distance travelled by the sample over the total distance covered by the solvent is referred to as ___________

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Q. _______________ is a liquid column chromatographic method of separating solute molecules according to differences in molecular size.

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Q. Which of the following technique separates the sample by percolating it through a bed of porous, uncharged particles?

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Q. Which of the following is not a step involved in gel filtration?

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Q. Which of the following is not a gel filtration media used in gel filtration?

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Q. Which gel filtration media is used to purify alpha amylase by gel filtration?

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Q. Alkaline protease is purified by gel filtration using __________ as gel filtration media.

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Q. Smaller molecules elute faster than larger molecules during gel filtration.

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Q. Pick the odd one out.

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Q. _____________ retains analyte molecules based on the coulombic interaction.

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Q. When pH of enzyme solution is below their isoelectric point, the enzymes will be negatively charged and bind to anion exchangers.

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