Q. _____________ retains analyte molecules based on the coulombic interaction.

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Q. When pH of enzyme solution is below their isoelectric point, the enzymes will be negatively charged and bind to anion exchangers.

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Q. Cation exchange chromatography retains positively charged cations.

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Q. Which of these is not an anion exchanger?

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Q. Pick the odd one out.

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Q. Peroxidases can be purified using ___________

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Q. Alkaline protease is purified by using ________

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Q. Cation exchange resin is used to purify __________

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Q. Which of the functional groups are not present in cation exchangers?

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Q. The chromatographic method of separating biochemical mixture of compounds, based on highly specific biological interactions is referred to as ______________

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Q. Which of the following is not a highly specific biological interaction to be used in affinity chromatography?

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Q. Which of this is not true while selecting a solid matrix or column during affinity chromatography?

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Q. In affinity chromatography, the matrix should be chemically and mechanically stable.

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Q. The matrix selected during affinity chromatography must possess certain chemical groups.

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Q. The first step in preparation of affinity chromatography column is _____________

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Q. __________ refers to eluting a compound by creating a competition between the bounded enzymes with its suitable complimentary protein in solution.

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Q. Which among these is not a way of non-specific elution?

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Q. Acetylcholine esterase can be purified by using affinity chromatography with the use of _______ as a ligand.

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Q. Acid phosphatase: Mannose:: Alpha amylase: ______________

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Q. The chromatography in which the sample is forced through a column of the stationary phase by pumping a liquid (mobile phase) at high pressure through the column is referred to as ___________

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