Q. In an experiment you use RNA polymerase without its sigma factor for transcription. What will be the result that you observe?

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Q. In an experiment you mutate the consensus sequence at the -10 box. You observe the rate of transcription reduces. Now you make complementary mutation to the sigma factor. What will you observe?

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Q. You wanted to isolate active RNA polymerase (with a part of the transcript associated with it ) from cellular mix. For this, you perform an immunoprecipitation with anti sigma antibody. Then you check the precipitate. What will you observe?

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Q. Which part of sigma factor associates with -10box?

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Q. You want to find out which of the consequent gene X or Y is being transcribed. For this you do a hybridization competition analysis and plot the radioactivity curve with increasing concentration of the competitor unlabelled mRNA. If the transcribed gene was X, which observation fits your result?

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Q. Which of these is the 1st event to take place during transcription initiation?

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Q. The catalytic unit of RNA polymerases when placed properly during initiation is just over _________

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Q. In an experiment you mutate the C terminal domain of alpha subunit of the RNA polymerase. What will you expect to see?

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Q. Mutations as far as 100 base pairs upstream to +1 site will also affect the transcription rate.

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Q. In an experiment you add increasing amount of sigma factor to a mixture of DNA fragment and core polymerase in vitro. You also add [¹⁴C]ATP and [γ⁻³²P]ATP in the mixture and check the rate of incorporation. Results show that the incorporation of both of this labels increase on adding more sigma factor. What will you conclude from your observation?

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Q. In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1st set you deleted 94 amino acids from the C-terminus and in the 2nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?

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Q. Which cation is placed in the catalytic subunit of RNA polymerase?

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Q. While studying the transcripts in vitro you observe that other than the longer transcripts there are some short stretches of RNA oligomer produced as well. You repeat the experiment several times but these oligomers seem to persist. What is the possible explanation?

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Q. Capping of RNA is necessary as_______

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Q. What is the 1st nucleotide of a completely synthesized transcript?

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Q. Processivity of RNA polymerase is ________

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Q. Which antibiotic inhibits transcription elongation?

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Q. You design a reconstructive RNA polymerase where you take the beta and alpha subunit from a rifampicin resistant strain and beta prime and sigma subunit from rifampicin sensitive strain. You check the rate of transcription by this RNA polymerase after the addition of the antibiotic. What will be your observation?

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Q. You add labeled Uridine analogue to the mixture of DNA and RNA pol in vitro. What are you trying to determine?

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Q. Which of these is not a part of RNA polymerase elongation machinery?

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