Q. You design a reconstructive RNA polymerase where you take the beta and alpha subunit from a rifampicin resistant strain and beta prime and sigma subunit from rifampicin sensitive strain. You check the rate of transcription by this RNA polymerase after the addition of the antibiotic. What will be your observation?

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Q. You add labeled Uridine analogue to the mixture of DNA and RNA pol in vitro. What are you trying to determine?

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Q. Which of these is not a part of RNA polymerase elongation machinery?

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Q. Melting of DNA would lead to _________________

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Q. In a stretch of DNA being transcribed, the region forward to the Elongation is __________

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Q. What form of helix would you expect to see in double stranded RNA?

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Q. A ssRNA shows inverted tandem repeat. What would be its secondary structure?

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Q. To study the functioning of intrinsic terminator you increase the number of G-C base pairs in the stem and decrease the U bases from the tail region. What would be its effect on termination?

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Q. Which of these interactions is the weakest?

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Q. You modify your gene DNA sequence by introducing an attenuator within the transcribed region. You isolate the labeled gene product and perform SDS PAGE. You observe two bands one higher and another slightly lower. Which of this band corresponds to properly modified sequence?

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Q. Rho protein, that is necessary for transcription termination, is a ________

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Q. Rho shows base preference although it doesn’t have any concensus cis binding sequence. Which region will be expected to bind Rho better?

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Q. Rho dependent termination mechanism doesn’t reguire____________

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Q. When Rho catches up with RNA polymerase it _______

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Q. You can see non-cannonical base pairing in________

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Q. RNA has a proofreading mechanism.

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Q. Which of these proteins help to backtrack the RNA?

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Q. If you study T4 bacteriophage transcription with holoenzyme which genes will you expect to be transcribed?

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Q. You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________

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Q. When sigma subunit dissociates from the RNA polymerase what happens to the DNA binding clamp of the core?

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