Q. Which of these proteins help to backtrack the RNA?

View solution

Q. If you study T4 bacteriophage transcription with holoenzyme which genes will you expect to be transcribed?

View solution

Q. You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________

View solution

Q. When sigma subunit dissociates from the RNA polymerase what happens to the DNA binding clamp of the core?

View solution

Q. In an experiment you wrongly added Rifampicin antibiotic to Rifampicin sensitive bacteria. You try to correct your mistake by adding more core subunits (resistant to Rifampicin) to your mixture. What would you expect to see?

View solution

Q. Which is the primary sigma factor in Bacillus subtilis?

View solution

Q. What is the function of 1st subunit of sigma factor in σ⁷⁰ and σ⁴³?

View solution

Q. Loosening the binding between polymerase and non-specific site is important to confer higher specificity. In Bacillus subtilis what is responsible for this action?

View solution

Q. We know that region 2.4 of sigma factor binds DNA at -10box. What is the function of 2.1?

View solution

Q. Which DNA binding domain is seen in region 4.2 of sigma factor?

View solution

Q. Which sigma factor is bound to Rse A?

View solution

Q. You artificially increase the level of σ³² in an E. coli culture. What will you expect to see?

View solution

Q. If a bacteria is facing lack of carbon sources in the media, which sigma factor concentration is expected to increase?

View solution

Q. You want your bacterial culture to grow well so you made an enriched media with all forms of carbohydrates. Which of this carbohydrate should you restore first if you want the culture to keep growing at the same rate?

View solution

Q. In a mero-diploid you have one I- and one I+ Repressor gene in the two lac operons. I+ is wild type while I- is a mutant which produces a repressor that doesn’t bind. What would be the functionality of the diploid?

View solution

Q. Which of these Lac operon mero- diploid will be constitutively turned on? Considering the terms has their usual meaning.

View solution

Q. Which of these combinations will result is am operon that can never produce beta galactosidse?

View solution

Q. Which of the Ars operon regulation site is actually preventing RNA polymerase from binding and producing the Ara genes BAD?

View solution

Q. Which of these Ara genes is a mode of feedback autoregulation?

View solution

Q. In which of this condition is Ara operon functional?

View solution