Q. You modify your gene DNA sequence by introducing an attenuator within the transcribed region. You isolate the labeled gene product and perform SDS PAGE. You observe two bands one higher and another slightly lower. Which of this band corresponds to properly modified sequence?

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Q. Rho protein, that is necessary for transcription termination, is a ________

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Q. Rho shows base preference although it doesn’t have any concensus cis binding sequence. Which region will be expected to bind Rho better?

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Q. Rho dependent termination mechanism doesn’t reguire____________

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Q. When Rho catches up with RNA polymerase it _______

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Q. You can see non-cannonical base pairing in________

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Q. RNA has a proofreading mechanism.

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Q. Which of these proteins help to backtrack the RNA?

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Q. If you study T4 bacteriophage transcription with holoenzyme which genes will you expect to be transcribed?

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Q. You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________

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Q. When sigma subunit dissociates from the RNA polymerase what happens to the DNA binding clamp of the core?

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Q. In an experiment you wrongly added Rifampicin antibiotic to Rifampicin sensitive bacteria. You try to correct your mistake by adding more core subunits (resistant to Rifampicin) to your mixture. What would you expect to see?

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Q. Which is the primary sigma factor in Bacillus subtilis?

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Q. What is the function of 1st subunit of sigma factor in σ⁷⁰ and σ⁴³?

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Q. Loosening the binding between polymerase and non-specific site is important to confer higher specificity. In Bacillus subtilis what is responsible for this action?

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Q. We know that region 2.4 of sigma factor binds DNA at -10box. What is the function of 2.1?

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Q. Which DNA binding domain is seen in region 4.2 of sigma factor?

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Q. Which sigma factor is bound to Rse A?

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Q. You artificially increase the level of σ³² in an E. coli culture. What will you expect to see?

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Q. If a bacteria is facing lack of carbon sources in the media, which sigma factor concentration is expected to increase?

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