Q. Mutations as far as 100 base pairs upstream to +1 site will also affect the transcription rate.

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Q. In an experiment you add increasing amount of sigma factor to a mixture of DNA fragment and core polymerase in vitro. You also add [¹⁴C]ATP and [γ⁻³²P]ATP in the mixture and check the rate of incorporation. Results show that the incorporation of both of this labels increase on adding more sigma factor. What will you conclude from your observation?

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Q. In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1st set you deleted 94 amino acids from the C-terminus and in the 2nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?

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Q. Which cation is placed in the catalytic subunit of RNA polymerase?

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Q. While studying the transcripts in vitro you observe that other than the longer transcripts there are some short stretches of RNA oligomer produced as well. You repeat the experiment several times but these oligomers seem to persist. What is the possible explanation?

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Q. Capping of RNA is necessary as_______

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Q. What is the 1st nucleotide of a completely synthesized transcript?

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Q. Processivity of RNA polymerase is ________

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Q. Which antibiotic inhibits transcription elongation?

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Q. You design a reconstructive RNA polymerase where you take the beta and alpha subunit from a rifampicin resistant strain and beta prime and sigma subunit from rifampicin sensitive strain. You check the rate of transcription by this RNA polymerase after the addition of the antibiotic. What will be your observation?

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Q. You add labeled Uridine analogue to the mixture of DNA and RNA pol in vitro. What are you trying to determine?

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Q. Which of these is not a part of RNA polymerase elongation machinery?

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Q. Melting of DNA would lead to _________________

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Q. In a stretch of DNA being transcribed, the region forward to the Elongation is __________

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Q. What form of helix would you expect to see in double stranded RNA?

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Q. A ssRNA shows inverted tandem repeat. What would be its secondary structure?

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Q. To study the functioning of intrinsic terminator you increase the number of G-C base pairs in the stem and decrease the U bases from the tail region. What would be its effect on termination?

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Q. Which of these interactions is the weakest?

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Q. You modify your gene DNA sequence by introducing an attenuator within the transcribed region. You isolate the labeled gene product and perform SDS PAGE. You observe two bands one higher and another slightly lower. Which of this band corresponds to properly modified sequence?

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Q. Rho protein, that is necessary for transcription termination, is a ________

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