Q. In a substrate X catabolic gene promoter you replace the normal histones by your experimental histones – ones with arginine side chains in place of histidine. What will be the effect on the concentration of substrate X against the control?

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Q. You take a crude cell lysate and treat it with DNase. Now wash it and add protease. Use specific restriction endonuclease to get your desired fragment and run it on a gel. You see a single band. Which of the following will be true about the fragment?

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Q. Which of the following is not a common mode of histone modification in eukaryotes?

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Q. Which of the following association acts as transcriptional activator?

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Q. In an experiment you perform the following- you attach a flag epitope to HDAC, you over express a mutated Mad1-pro gene within the experimental cell, you immunoprecipitate the protein complex using flag epitope and run that on a non-denaturing SDS PAGE and western blot. Now you add antibodies for HDAC, MAD1 and SIN3A stripping after each addition. What will you observe?

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Q. In presence of Thyroid hormone the receptor TR binds to ____________

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Q. If the events in post transcriptional modification are listed as-

i) Polyadenylation ii) capping iii) splicing

What would be the correct sequence?

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Q. Which RNA transcript would be capped by the capping enzymes in vitro mixture of RNA?

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Q. Which nucleotide is present in the 5’ cap?

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Q. Which is the 1st enzyme in capping?

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Q. The post transcriptional modifications are done after the transcript is completely transcribed. State whether the statement is true or false.

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Q. CBP acts as a(an)_______________

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Q. In an experiment you try to hybridize a DNA single strand with a mature RNA. You observe loops being formed. These loops have ____________

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Q. Which of the following corresponding to the same gene will you expect to be the shortest?

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Q. Splicing concensus sequence is ___________

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Q. In the consensus sequence 5′-AG/GUAAGU–intron–YNCURAC–YnNAG/G-3′ Y could be ______

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Q. In an experiment, you are trying to pull down the site where guanyl transferase binds by co-immunoprecipitation. You run a gel and autoradiograph to see a band denoting GSK-RNA pol II (phosphorylated). What will you see when you perform the same experiment with overexpression of GSK in one setup and overexpression of unlabelled GSK-RNA pol II in the other setup?

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Q. The poly a tail protects the 3’ end from____________

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Q. Which of the following mRNA will not be polyadenylated?

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Q. Which of the box is retained in mature mRNA?

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