Q. At times, a specific fragment of the molecules of DNA which are analyzed needs to be separated. One of the methods is digesting the gels simply. Which of the following statement is not correct in with respect to it?

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Q. UV shadowing is used at times for visualising the DNA. Which of the statements is correct for it?

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Q. The greatest separation is obtained in which portion of the gel?

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Q. Which of the statements is correct for non uniform separation obtained other than the conventional one?

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Q. How can gradient in the field strength be obtained?

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Q. If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used?

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Q. Which of the following statement is correct for the method of separation of large molecules?

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Q. How many approaches are there in order to clone the complete genome?

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Q. If a full proteomic analysis of growth medium is carried out and is combined with ____________ genome sequence, genes for other _____________ proteins are also obtained.

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Q. If a putative protein sequence is cloned in an expression vector and the expressed protein is not showing protease activity, then which of the following is not correct?

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Q. For getting a large amount of proteins to crystallize, which of the following should be used as an expression system?

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Q. If a mutation perturbs the structure, then stability and folding are not affected.

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Q. The RNA level ___________ the steady-state level of the corresponding protein directly and the post-translational modification of the protein ____________

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Q. For a convenient transformation system, _____ can be used for gene silencing.

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Q. The term ‘endonuclease’ refers to cutting the DNA sequence from ______________

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Q. The restriction endonuclease is having a defence mechanism in the bacterial system against foreign DNA such as viruses. But how it is able to protect its own DNA?

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Q. Even after replication, how the modified DNA remains protected?

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Q. How many classes of restriction enzymes are there?

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Q. Type II cuts the sequence in the following way __________

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Q. After cleaving the sequence, the nature of the ends created by the type II endonuclease is __________

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