Question

After carrying out the cloning experiment, the cells are plated on agar. Along with agar, it also contains antibiotic resistant genes, X-gal and an inducer of lacZ gene. Which of the following would grow?

a.

Cells that have taken up plasmid DNA

b.

Cells that have taken up genomic DNA

c.

Cells having no insert

d.

Cells having no insert

Posted under Genetic Engineering

Answer: (a).Cells that have taken up plasmid DNA

Interact with the Community - Share Your Thoughts

Uncertain About the Answer? Seek Clarification Here.

Understand the Explanation? Include it Here.

Q. After carrying out the cloning experiment, the cells are plated on agar. Along with agar, it also contains antibiotic resistant genes, X-gal and an inducer of lacZ gene. Which of...

Similar Questions

Explore Relevant Multiple Choice Questions (MCQs)

Q. Choose the correct statement for adaptors.

Q. If linkers are combined with other features such as a selectable marker, it is called as __________

Q. Plasmids are used for carrying out the cloning procedure. Which of the statement is true for plasmids?

Q. Which of the following characteristic is not present in a plasmid on a general basis?

Q. Bla is a gene, which is incorrect for it?

Q. Which of the characteristics is present in lacZ gene?

Q. Which of the following orientation is not propagated to later stages?

Q. Molecules having new combination of sequences that were not present before are called as __________

Q. Insertion of DNA into lacZ gene may lead to disruption of the gene function.

Q. Which of the following is true regarding taking up of plasmid DNA in the bacterial cells?

Q. If genomic DNA has been inserted instead of the plasmid, what will happen?

Q. Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________

Q. Generation of recombinants by randomly cloning fragments of total DNA from an organism is called as __________

Q. The phenomenon of alpha complementation is __________

Q. An operon is defined as __________

Q. Why the whole lacZ gene can’t be present in the plasmid at one time?

Q. Alpha complementation is an indicator of lacZ system. Which of the statement is incorrect for it?

Q. What will be the consequence of not having an origin of replication (ori) in the vector?

Q. It is required to distinguish between the cells that have taken up the vector and that have not. It is done by using __________

Q. Multiple cloning site (MCS) is defined as __________

Recommended Subjects

Are you eager to expand your knowledge beyond Genetic Engineering? We've handpicked a range of related categories that you might find intriguing.

Click on the categories below to discover a wealth of MCQs and enrich your understanding of various subjects. Happy exploring!