Question

Which of the following is a restriction for cloning in bacteriophage Mu?

a.

It is limited to handling only small number of phage particles

b.

It relies on whether the DNA is flanked by two mini Mu genes or not

c.

Recombination between the elements in the second strain is not a restriction

d.

Fragments of any genomic DNA can be cloned irrespective of the fact whether or not the phage can replicate in it or not

Answer: (b).It relies on whether the DNA is flanked by two mini Mu genes or not

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Q. Which of the following is a restriction for cloning in bacteriophage Mu?

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