Q. Thioredexin protein contains two _______ residues.

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Q. Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.

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Q. Pel B protein is produced in plants and helps in the degradation of ______

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Q. His tagged proteins can be eluted using EDTA or a pH gradient from the matrix.

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Q. Enterokinase is an intestinal enzyme that converts _______ to ________

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Q. Cyanogen bromide is used for cleavage of junctions. It cleaves after ________ residues.

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Q. Cloning vectors designed for the purpose of synthesis of RNA and proteins are known as ___________

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Q. For studying processes such as splicing and cleavage, RNA is required. Choose the correct statement for this.

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Q. RNA can be synthesized by using vector. A vector with _______ is used and further through ________ RNA is isolated.

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Q. Isolation of RNA can be carried out easily from bacterial cells.

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Q. Promoters are generally used after isolation from ___________

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Q. By selecting the appropriate polymerase to activate the promoter ________ can be carried out _______

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Q. Guanylyl transferase and GTP are used for?

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Q. Capping can be introduced by the use of cap analogue. Which of the statement is true?

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Q. If transcription should not be carried out beyond the insert in the vector, then it should be linearized.

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Q. If the cells containing plasmids are infected with helper phage, which type of DNA can be produced, packaged and secreted into the medium?

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Q. Little quantities of radiolabelled proteins are required for which of the following?

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Q. __________ quantities of _______ protein are required for determination of properties in biochemical and biological assays.

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Q. Small quantities of radiolabelled RNA can be produced by translation in vitro which is done by transcription in vitro.

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Q. For the production of unlabelled and huge amount of proteins, which of the following is true?

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