Q. For studying processes such as splicing and cleavage, RNA is required. Choose the correct statement for this.

View solution

Q. RNA can be synthesized by using vector. A vector with _______ is used and further through ________ RNA is isolated.

View solution

Q. Isolation of RNA can be carried out easily from bacterial cells.

View solution

Q. Promoters are generally used after isolation from ___________

View solution

Q. By selecting the appropriate polymerase to activate the promoter ________ can be carried out _______

View solution

Q. Guanylyl transferase and GTP are used for?

View solution

Q. Capping can be introduced by the use of cap analogue. Which of the statement is true?

View solution

Q. If transcription should not be carried out beyond the insert in the vector, then it should be linearized.

View solution

Q. If the cells containing plasmids are infected with helper phage, which type of DNA can be produced, packaged and secreted into the medium?

View solution

Q. Little quantities of radiolabelled proteins are required for which of the following?

View solution

Q. __________ quantities of _______ protein are required for determination of properties in biochemical and biological assays.

View solution

Q. Small quantities of radiolabelled RNA can be produced by translation in vitro which is done by transcription in vitro.

View solution

Q. For the production of unlabelled and huge amount of proteins, which of the following is true?

View solution

Q. How many methods are there, which are used for protein synthesis in vitro?

View solution

Q. Reticulocytes are immature red cells, which are obtained from ___________

View solution

Q. What is the use of adding micrococcal nuclease in the reticulocyte cells?

View solution

Q. Treatment of reticulocyte cells is done with EGTA. It chelates the calcium ions which are required for functioning of micrococcal nuclease.

View solution

Q. In ________ transcription and translation is coupled.

View solution

Q. In which of the following systems, transcription and translation are carried out together?

View solution

Q. If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid.

View solution