Q. At times it is observed that non-recombinant plasmids are more than that of recombinant plasmids. Choose the correct statement in regard to it.

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Q. Alkaline Phosphatase is used at times and the vector is treated with it. Choose the incorrect statement.

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Q. What is the correct time for carrying out the alkaline phosphatase treatment?

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Q. ccdB gene is used at times. Choose the correct statement with respect to this gene.

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Q. When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________

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Q. What happens if insert DNA is cut with two different restriction enzymes at the ends?

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Q. What is the disadvantage of amplification of using PCR over natural cloning?

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Q. TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it?

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Q. Choose the incorrect statement in respect to topoisomerase I.

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Q. Linkers are often used in cloning. Choose the incorrect statement for linkers.

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Q. There is no effect if the insert itself contains the restriction site.

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Q. For cloning, DNA samples are taken from

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Q. A small circular DNA present in bacterial cells are called as

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Q. DNA extracted from an organism is cut into gene size pieces with

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Q. Library screening identify colonies which have specific

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Q. A collection of total genomic DNA from a single organism is called

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Q. The enzyme that cut the DNA at or near restriction sites are called as

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Q. The process in which the bacterial cell wall is disrupted by using small electric pulses is

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Q. Gene mapping provides useful information about chance of

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Q. The methods used to identify the locus of the gene and distances between genes are called as

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