Q. A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

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Q. Choose the correct statement.

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Q. PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

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Q. Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.

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Q. If PCR is used to introduce random mutations rather than specific mutations, it is called as ___________

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Q. For the selection of the molecules having mutated sequence, which of the statement is true?

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Q. Choose the incorrect statement for the methodology of selection of molecules with mutant sequences.

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Q. Once second strand synthesis is carried out, it is introduced into the host. Host is having which mutation?

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Q. Replication by first strand leads to the formation of mutated molecules and functional antibiotic resistant gene.

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Q. In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?

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Q. What is the function of ung gene?

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Q. DpnI cuts ________ strands.

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Q. The minor change in amino acid sequence can lead to _______ effect on three dimensional structure and there _____ in the primary sequence.

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Q. It is often useful to inactivate endogenous genes in an organism. It might be helpful in finding out _________ role of the wild type gene.

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Q. The inactivation of endogenous genes may also be helpful in directing the expression of the mutated gene in the absence of background expression of wild type gene.

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Q. How can mutant strains be produced?

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Q. The principle of gene disruption is ________ to replace the endogenous chromosomal copy of a gene with __________

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Q. The gene to be disrupted is cloned and a selectable marker is inserted. What should be the effect of selectable marker?

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Q. The disrupted gene is excised from the vector and is inserted into the target organism. The excised gene should be in which form?

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Q. Stable acquisition of the marker can take place only if a double crossover over the flanking sequence and their chromosomal counterparts causes the marker’s integration into the chromosome.

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