Q. Replication by first strand leads to the formation of mutated molecules and functional antibiotic resistant gene.

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Q. In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?

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Q. What is the function of ung gene?

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Q. DpnI cuts ________ strands.

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Q. The minor change in amino acid sequence can lead to _______ effect on three dimensional structure and there _____ in the primary sequence.

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Q. It is often useful to inactivate endogenous genes in an organism. It might be helpful in finding out _________ role of the wild type gene.

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Q. The inactivation of endogenous genes may also be helpful in directing the expression of the mutated gene in the absence of background expression of wild type gene.

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Q. How can mutant strains be produced?

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Q. The principle of gene disruption is ________ to replace the endogenous chromosomal copy of a gene with __________

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Q. The gene to be disrupted is cloned and a selectable marker is inserted. What should be the effect of selectable marker?

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Q. The disrupted gene is excised from the vector and is inserted into the target organism. The excised gene should be in which form?

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Q. Stable acquisition of the marker can take place only if a double crossover over the flanking sequence and their chromosomal counterparts causes the marker’s integration into the chromosome.

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Q. If ______ gene is there, the double crossover may leave ________ in the chromosome.

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Q. If the target organism contains more than one copy of the gene, what is the effect on these copies?

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Q. If methods are based on cellular processes that lead to inactivation of gene expression by affecting the RNA, then it is called as ___________

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Q. Choose the incorrect statement for the method based on antisense RNA.

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Q. The method of post transcriptional gene silencing is particularly useful in ___________

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Q. Down regulation of expression of endogenous genes by transformation with constructs that would generate sense RNA, rather than anti-sense RNA is known as ___________

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Q. In some organisms, presence of double stranded RNAs leads to breakdown of corresponding single stranded mRNA.

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Q. Double stranded RNA is cleaved by a nuclease called as Dicer and small fragments are generated known as ___________

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