Q. Cells mutant in _____ gene can be selected by using 5-fluoro orotic acid, which is turned into ____ products by wild type protein.

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Q. The replicating plasmid vectors in yeast can be divided broadly into how many categories?

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Q. Yeast centromeric plasmid (YCp50) contains ampicillin and tetracycline resistant gene.

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Q. CEN4 in YCp50 is an example of _______

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Q. Yeast episomal plasmids have the following feature?

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Q. Cis-acting REP 3 sequence is present in Yeast episomal plasmids. Choose the statement which holds true for it?

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Q. When YAC is used to clone DNA. What is the size of the DNA that can be cloned?

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Q. TEL is the ______ sequence derived from the ends of ribosomal RNA-encoding molecules from the macromolecules of the protozoan Tetrahyena.

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Q. Choose the incorrect statement for YAC vectors.

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Q. If YAC DNA is digested with BamHI and ECoRI, how many fragments are generated?

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Q. Transformed cells in the case of YAC can be selected by the presence of both URA3 and TRP1 genes.

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Q. GAL1 is a controllable promoter and is obtained from genes for galactose metabolism. It is known for encoding ______

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Q. Transcription of GAL1 and GAL10 is suppressed in the presence of _______

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Q. How many deletion variations of GAL1 promoter are there?

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Q. Promoters such as GAL1 can be used to direct synthesis of either unmodified or fusion proteins.

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Q. Which of the following are advantageous to be used for expression of eukaryotic cells?

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Q. Secretion of overexpressed proteins by fusion to a secretion signal can be useful because it helps in bringing about correct _______ and _____ protein folding.

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Q. The enzyme used for breaking down of sucrose into glucose and fructose is called as _______________

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Q. For introduction of exogenous DNA into S.cerevisiae ______ is added along with polyethylene glycol and heat shock treatment.

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Q. Protoplast transformation is less time consuming than lithium acetate for transformation.

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