Q. Transcription of GAL1 and GAL10 is suppressed in the presence of _______

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Q. How many deletion variations of GAL1 promoter are there?

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Q. Promoters such as GAL1 can be used to direct synthesis of either unmodified or fusion proteins.

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Q. Which of the following are advantageous to be used for expression of eukaryotic cells?

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Q. Secretion of overexpressed proteins by fusion to a secretion signal can be useful because it helps in bringing about correct _______ and _____ protein folding.

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Q. The enzyme used for breaking down of sucrose into glucose and fructose is called as _______________

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Q. For introduction of exogenous DNA into S.cerevisiae ______ is added along with polyethylene glycol and heat shock treatment.

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Q. Protoplast transformation is less time consuming than lithium acetate for transformation.

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Q. Schizosaccharomyces pombe is yeast and it reproduces by __________

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Q. The leu1+ marker encodes which enzyme?

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Q. The replacement of one plasmid by another by introduction of the second plasmid into strain containing the first, followed by removal of the first by counterselection is known as __________

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Q. Promoters can be divided basically into how many types?

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Q. nmt1+ gene is used for _____ biosynthesis.

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Q. The promoter from fbp1+ gene is suppressed in the presence of glucose.

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Q. Species which can grow on _______ as a carbon source are called as methylotropic.

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Q. Chlamyodomonas reinhardtii is a green _________ algae.

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Q. Chlamyodomonas genome is _______ rich.

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Q. Integration of DNA into the Chlamydomonas genome is via homologous recombination.

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Q. The disease crown gall is caused by which bacteria?

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Q. Agrobacterium tumefaciens form ______ plasmids and Agrobacterium rhizogenes form _______ plasmids.

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