Mapping and Sequencing of Genomes MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Mapping and Sequencing of Genomes, a fundamental topic in the field of Cytogenetics. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Mapping and Sequencing of Genomes MCQs are designed to help you grasp the core concepts and excel in solving problems.

In this section, you'll find a wide range of Mapping and Sequencing of Genomes mcq questions that explore various aspects of Mapping and Sequencing of Genomes problems. Each MCQ is crafted to challenge your understanding of Mapping and Sequencing of Genomes principles, enabling you to refine your problem-solving techniques. Whether you're a student aiming to ace Cytogenetics tests, a job seeker preparing for interviews, or someone simply interested in sharpening their skills, our Mapping and Sequencing of Genomes MCQs are your pathway to success in mastering this essential Cytogenetics topic.

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Mapping and Sequencing of Genomes MCQs | Page 4 of 7

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Q31.
Which gene in a plasmid is indispensible for it to participate in conjugation?
Discuss
Answer: (c).Sex determining gene
Q32.
Which of the following can transfer the genes in bacterial nucleoid as well?
Discuss
Answer: (d).HFR
Q33.
A HFR bacteria mates with an F- plasmid containing bacteria. What will be the type of bacteria after conjugation is complete?
Discuss
Answer: (b).F-
Q34.
All the genes in the HFR that is transferred to the recipient cell before breakage of the conjugation channel is incorporated in the recipient genome. State whether it is true or false.
Discuss
Answer: (b).False
Q35.
In and interrupted mating experiment, it is seem that the after 10 mins Aziᴿ gene in recipient, after 20 minuites there is appearance of tonᴿ and after 15 mins the recipient is lac⁺ , but the recipient is almost never his⁺. Where in the genome is the ori of transfer?
Discuss
Answer: (d).Between His⁺ and Aziᴿ
Q36.
If genes enter the different F- cells from different HFRs in the order as shown-

Azi 8 88

Ton 10 90

Lac 17 3

Gal 24 11

What is the order of the gene where columns are for different HFR and values denote time?
Discuss
Answer: (b).Lac, Gal, Azi, Ton
Q37.
In an experiment of determining the order of gene in bacteria we use HFR which is gal+, Lac+, Ara+ and His+. If the recipient is negative for all these genes, and we selectively choose Gal + and get the pattern-

100 % recipients are Gal +
30% are His+
55% are Lac +
And only 5% are Ara+

What would be our inference?
Discuss
Answer: (a).Sequence of gene is Galβ€”Lacβ€”Hisβ€”Ara
Q38.
For complete HFR to enter the recipient, how much time should the two bacterial cells be in contact?
Discuss
Answer: (d).100 mins
Q39.
You study the gene incorporation in recipient using HFR and get the following sequences-
P-W-A-K, W-L-I-K, A-K-I-L, W-P-R-T-W-L. What is the order of the gene?
Discuss
Answer: (c).A-K-I-L-W-T-R-P-W
Q40.
In an experiment you have a U-Tube with two strains of bacteria in two arms. In one arm the bacteria are Gal+ and His-, in other the strain is Gal- and His+. If the two arms are joined by a membrane that doesn’t allow bacterial cells to pass to opposite site what will you observe when you plate each arm bacterial strain on minimal media?
Discuss
Answer: (d).Some colonies seen in both arms
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