Transcription in Prokaryotes and Eukaryotes MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Transcription in Prokaryotes and Eukaryotes, a fundamental topic in the field of Cytogenetics. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Transcription in Prokaryotes and Eukaryotes MCQs are designed to help you grasp the core concepts and excel in solving problems.

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Transcription in Prokaryotes and Eukaryotes MCQs | Page 1 of 16

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Q1.
Which protein mentioned below can reverse central dogma?

a.

Ribosome

b.

Restriction Endonuclease

c.

Reverse Transcriptase

d.

RNA Polymerase

Discuss
Answer: (c).Reverse Transcriptase
Q2.
The catalytic activity resides in which subunit of RNA polymerase?

a.

β′ (156 kDa)

b.

β (151 kDa)

c.

α (37 kDa)

d.

σ70 (70 kDa)

Discuss
Answer: (a).β′ (156 kDa)
Q3.
What is the direction in which the transcript produced by RNA polymerase grows?

a.

3’->5’ direction on 3’->5’ strand

b.

5’->3’ direction on 5’->3’ strand

c.

3’->5’ direction on 5’->3’ strand

d.

5’->3’ direction on 3’->5’ strand

Discuss
Answer: (d).5’->3’ direction on 3’->5’ strand
Q4.
In an experiment you use RNA polymerase without its sigma factor for transcription. What will be the result that you observe?

a.

More transcription

b.

Less transcription

c.

More specific transcription

d.

More random transcription

Discuss
Answer: (d).More random transcription
Q5.
In an experiment you mutate the consensus sequence at the -10 box. You observe the rate of transcription reduces. Now you make complementary mutation to the sigma factor. What will you observe?

a.

Further decrease in transcription rate

b.

Increase in transcription rate

c.

Original transcription rate

d.

Increase in rate of random transcription

Discuss
Answer: (c).Original transcription rate
Q6.
You wanted to isolate active RNA polymerase (with a part of the transcript associated with it ) from cellular mix. For this, you perform an immunoprecipitation with anti sigma antibody. Then you check the precipitate. What will you observe?

a.

RNA polymerase with transcript

b.

RNA polymerase without transcript

c.

Only antibody

d.

DNA with associated RNA polymerase and a transcript

Discuss
Answer: (b).RNA polymerase without transcript
Q7.
Which part of sigma factor associates with -10box?

a.

1.2

b.

2.4

c.

4.4

d.

4.2

Discuss
Answer: (b).2.4
Q8.
You want to find out which of the consequent gene X or Y is being transcribed. For this you do a hybridization competition analysis and plot the radioactivity curve with increasing concentration of the competitor unlabelled mRNA. If the transcribed gene was X, which observation fits your result?

a.

The radioactivity of y decreases while that of x remains the same

b.

The radioactivity of x decreases while that of y remains the same

c.

The radioactivity of x and y decreases

d.

The radioactivity of y decreases while x increases

Discuss
Answer: (b).The radioactivity of x decreases while that of y remains the same
Q9.
Which of these is the 1st event to take place during transcription initiation?

a.

Formation of a closed initiation complex

b.

Formation of open initiation complex

c.

Formation of absorptive transcript

d.

Promoter clearance

Discuss
Answer: (b).Formation of open initiation complex
Q10.
The catalytic unit of RNA polymerases when placed properly during initiation is just over _________

a.

-1 site

b.

0 site

c.

+1 site

d.

– 10 site

Discuss
Answer: (c).+1 site
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