Transcription in Prokaryotes and Eukaryotes MCQs

Welcome to our comprehensive collection of Multiple Choice Questions (MCQs) on Transcription in Prokaryotes and Eukaryotes, a fundamental topic in the field of Cytogenetics. Whether you're preparing for competitive exams, honing your problem-solving skills, or simply looking to enhance your abilities in this field, our Transcription in Prokaryotes and Eukaryotes MCQs are designed to help you grasp the core concepts and excel in solving problems.

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Transcription in Prokaryotes and Eukaryotes MCQs | Page 2 of 16

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Q11.
In an experiment you mutate the C terminal domain of alpha subunit of the RNA polymerase. What will you expect to see?

a.

Transcription is absent

b.

Transcription is at random sites

c.

Transcription is less

d.

Transcription is more

Discuss
Answer: (c).Transcription is less
Q12.
Mutations as far as 100 base pairs upstream to +1 site will also affect the transcription rate.

a.

True

b.

False

c.

May be True or False

d.

Can't say

Discuss
Answer: (a).True
Q13.
In an experiment you add increasing amount of sigma factor to a mixture of DNA fragment and core polymerase in vitro. You also add [¹⁴C]ATP and [γ⁻³²P]ATP in the mixture and check the rate of incorporation. Results show that the incorporation of both of this labels increase on adding more sigma factor. What will you conclude from your observation?

a.

Sigma confers specificity and increases transcription rate

b.

Sigma increases initiation and elongation rate

c.

Sigma increases initiation of transcription rate

d.

Sigma causes faster elongation

Discuss
Answer: (b).Sigma increases initiation and elongation rate
Q14.
In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1st set you deleted 94 amino acids from the C-terminus and in the 2nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?

a.

94 amino acid mutant

b.

265th amino acid mutant

c.

Both will have similar negative effect

d.

No visible effect on rate of transcription

Discuss
Answer: (c).Both will have similar negative effect
Q15.
Which cation is placed in the catalytic subunit of RNA polymerase?

a.

Zn²⁺

b.

Mn²⁺

c.

Mg²⁺

d.

Fe³⁺

Discuss
Answer: (c).Mg²⁺
Q16.
While studying the transcripts in vitro you observe that other than the longer transcripts there are some short stretches of RNA oligomer produced as well. You repeat the experiment several times but these oligomers seem to persist. What is the possible explanation?

a.

Procedural error

b.

They are the un-ligated oligomers of lagging strand

c.

Experimental artifact

d.

Normal occurrence

Discuss
Answer: (c).Experimental artifact
Q17.
Capping of RNA is necessary as_______

a.

It helps us distinguish 5’ from 3’ end

b.

It has a rolling action and condenses the transcript as it is produced

c.

To protect the transcript from exonuclease

d.

To prevent the transcript from sticking to DNA

Discuss
Answer: (c).To protect the transcript from exonuclease
Q18.
What is the 1st nucleotide of a completely synthesized transcript?

a.

A

b.

G

c.

C

d.

T

Discuss
Answer: (b).G
Q19.
Processivity of RNA polymerase is ________

a.

Very high

b.

High

c.

Low

d.

Very low

Discuss
Answer: (a).Very high
Q20.
Which antibiotic inhibits transcription elongation?

a.

Rifampicin

b.

Streptolydigin

c.

Penicillin

d.

Tetracyclin

Discuss
Answer: (b).Streptolydigin
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