Question
a.
enzyme immobilization
b.
adsorption
c.
biosensor
d.
carrier matrix
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Q. The surface of the matrix on which an enzyme is immobilized is called __________
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Q. Pick the odd one out.
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Q. Which of the following is not an example of adsorbent?
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Q. The immobilization method in which the enzyme is linked to the carrier matrix by covalent bond between them is referred to as __________
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Q. In which type of immobilization, the enzyme will be in a molecular cage?
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Q. In which of the following method, entrapment can be achieved?
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Q. In ________ method, the enzyme molecule in aqueous solution is confined within a semipermeable membrane.
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Q. The kinetic behavior of a bound enzyme is similar to that of free enzyme.
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Q. For positively charged substrates, \(K_m^{app}\) is given by which of the following?
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Q. Effectiveness factor is given by __________
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Q. Immobilization can greatly affect the stability of the enzyme.
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Q. The ratio of volume of solution contained within the particle to the total volume of the particle is referred to as ____________
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Q. What does the equation, λ=\(\frac{[C_0^{n+}]}{[C^{n+}]}=\frac{[A^{n-}]}{[A_0^{n-}]}\) represent?
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Q. Which of the following equation is true for \(K_m^{app}\), when the substrates are negatively charged?
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Q. Immobilized enzymes observe diffusional resistance at different levels.
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Q. In case of immobilized enzymes, what type of inhibition is represented by the following equation?
\(V=\frac{V_{max} S}{K_m+\frac{K_m [S_0]K_L^s}{K_p K_l^p}}\)
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Q. Immobilization affects the kinetic constants (Kₘ and Vₘₐₓ) of the enzyme.
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Q. __________ is the average ratio of path length between any points within the particle to their absolute distance apart.
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Q. What does the following equation represent?
V=\(\frac{V_{max}}{1+\frac{K_m^{app}}{[S_0]}}\)
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Q. What does the equation, \(D_a = \frac{V_{max}}{K_L [S_b]}\) represent?
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Q. In case of immobilized enzymes, the equation \(V=K_L ([S_0]-[S])=\frac{V_{max} [S]}{K_m+S+\frac{S^2}{K_S}}\) represents ______________ inhibition.
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