Question

The immobilization method in which the enzyme is linked to the carrier matrix by covalent bond between them is referred to as __________

a.

adsorption

b.

covalent binding

c.

entrapment

d.

membrane confinement

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Answer: (b).covalent binding

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Q. The immobilization method in which the enzyme is linked to the carrier matrix by covalent bond between them is referred to as __________

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Q. In which type of immobilization, the enzyme will be in a molecular cage?

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Q. The kinetic behavior of a bound enzyme is similar to that of free enzyme.

Q. For positively charged substrates, \(K_m^{app}\) is given by which of the following?

Q. Effectiveness factor is given by __________

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Q. What does the equation, λ=\(\frac{[C_0^{n+}]}{[C^{n+}]}=\frac{[A^{n-}]}{[A_0^{n-}]}\) represent?

Q. Which of the following equation is true for \(K_m^{app}\), when the substrates are negatively charged?

Q. Immobilized enzymes observe diffusional resistance at different levels.

Q. In case of immobilized enzymes, what type of inhibition is represented by the following equation?

\(V=\frac{V_{max} S}{K_m+\frac{K_m [S_0]K_L^s}{K_p K_l^p}}\)

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Q. What does the following equation represent?

V=\(\frac{V_{max}}{1+\frac{K_m^{app}}{[S_0]}}\)

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Q. Within the micro-environment, the same bulk concentration may give two different stable concentrations.

Q. What does the following diagram represents?

Q. The following diagram represents ____________ method of immobilization.

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