Q. The –35 sequence is highly conserved and has a consensus sequence of ___________

View solution

Q. The +1 site of conserved promoter sequence is generally a pyrimidine.

View solution

Q. –10, – 35 and +1 sites are the consensus promoter sites of sigma factor ______________

View solution

Q. The RNA polymerase core enzyme has a high specificity for promoter sites and can perfectly work alone.

View solution

Q. The complex formed by the polymerase and the promoter DNA is known as the _____________

View solution

Q. Negative supercoiling enhances the rate of transcription.

View solution

Q. The –10 promoter region is rich in ______________ bases.

View solution

Q. Without using primers for RNA synthesis the polymerase produces small stretches of RNA of _____________ nucleotides.

View solution

Q. To facilitate binding of σ⁷⁰ the melting of the template and non – template strands occurs between the positions ____________

View solution

Q. The isomerisation of σ⁷⁰ of bacterial polymerase is an energy dependent process.

View solution

Q. With respect to the formation of a complex between polymerase and DNA template which of the following is correct?

View solution

Q. How many channels are found in the RNA polymerase clamp?

View solution

Q. The channel that allows the exit of the coding strand is known as ____________

View solution

Q. Region 1.1 of the σ factor mimics the DNA and is negatively charged.

View solution

Q. RNA polymerase adds the nucleotides de novo. The first nucleotide added by the polymerase is ____________

View solution

Q. The ternary complex is formed by the polymerase, DNA duplex and the nascent RNA.

View solution

Q. After the binding of polymerase to the DNA template the σ factor is ejected.

View solution

Q. How many nucleotides of the growing RNA chain remain base paired with the template DNA?

View solution

Q. The transcription bubble formed for facilitating RNA synthesis is about _____________ nucleotides long.

View solution

Q. The nascent RNA forms a double helix with the non – coding strand.

View solution