Q. The +1 site of conserved promoter sequence is generally a pyrimidine.

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Q. –10, – 35 and +1 sites are the consensus promoter sites of sigma factor ______________

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Q. The RNA polymerase core enzyme has a high specificity for promoter sites and can perfectly work alone.

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Q. The complex formed by the polymerase and the promoter DNA is known as the _____________

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Q. Negative supercoiling enhances the rate of transcription.

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Q. The –10 promoter region is rich in ______________ bases.

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Q. Without using primers for RNA synthesis the polymerase produces small stretches of RNA of _____________ nucleotides.

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Q. To facilitate binding of σ⁷⁰ the melting of the template and non – template strands occurs between the positions ____________

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Q. The isomerisation of σ⁷⁰ of bacterial polymerase is an energy dependent process.

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Q. With respect to the formation of a complex between polymerase and DNA template which of the following is correct?

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Q. How many channels are found in the RNA polymerase clamp?

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Q. The channel that allows the exit of the coding strand is known as ____________

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Q. Region 1.1 of the σ factor mimics the DNA and is negatively charged.

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Q. RNA polymerase adds the nucleotides de novo. The first nucleotide added by the polymerase is ____________

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Q. The ternary complex is formed by the polymerase, DNA duplex and the nascent RNA.

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Q. After the binding of polymerase to the DNA template the σ factor is ejected.

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Q. How many nucleotides of the growing RNA chain remain base paired with the template DNA?

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Q. The transcription bubble formed for facilitating RNA synthesis is about _____________ nucleotides long.

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Q. The nascent RNA forms a double helix with the non – coding strand.

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Q. How many proof-reading activities does RNA polymerase have?

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