Q. Which histone(s) are acted upon by HAT?

View solution

Q. In a substrate X catabolic gene promoter you replace the normal histones by your experimental histones – ones with arginine side chains in place of histidine. What will be the effect on the concentration of substrate X against the control?

View solution

Q. You take a crude cell lysate and treat it with DNase. Now wash it and add protease. Use specific restriction endonuclease to get your desired fragment and run it on a gel. You see a single band. Which of the following will be true about the fragment?

View solution

Q. Which of the following is not a common mode of histone modification in eukaryotes?

View solution

Q. Which of the following association acts as transcriptional activator?

View solution

Q. In an experiment you perform the following- you attach a flag epitope to HDAC, you over express a mutated Mad1-pro gene within the experimental cell, you immunoprecipitate the protein complex using flag epitope and run that on a non-denaturing SDS PAGE and western blot. Now you add antibodies for HDAC, MAD1 and SIN3A stripping after each addition. What will you observe?

View solution

Q. In presence of Thyroid hormone the receptor TR binds to ____________

View solution

Q. If the events in post transcriptional modification are listed as-

i) Polyadenylation ii) capping iii) splicing

What would be the correct sequence?

View solution

Q. Which RNA transcript would be capped by the capping enzymes in vitro mixture of RNA?

View solution

Q. Which nucleotide is present in the 5’ cap?

View solution

Q. Which is the 1st enzyme in capping?

View solution

Q. The post transcriptional modifications are done after the transcript is completely transcribed. State whether the statement is true or false.

View solution

Q. CBP acts as a(an)_______________

View solution

Q. In an experiment you try to hybridize a DNA single strand with a mature RNA. You observe loops being formed. These loops have ____________

View solution

Q. Which of the following corresponding to the same gene will you expect to be the shortest?

View solution

Q. Splicing concensus sequence is ___________

View solution

Q. In the consensus sequence 5′-AG/GUAAGU–intron–YNCURAC–YnNAG/G-3′ Y could be ______

View solution

Q. In an experiment, you are trying to pull down the site where guanyl transferase binds by co-immunoprecipitation. You run a gel and autoradiograph to see a band denoting GSK-RNA pol II (phosphorylated). What will you see when you perform the same experiment with overexpression of GSK in one setup and overexpression of unlabelled GSK-RNA pol II in the other setup?

View solution

Q. The poly a tail protects the 3’ end from____________

View solution

Q. Which of the following mRNA will not be polyadenylated?

View solution