Question
i) Attaching of primers by cooling
ii) Denaturation of strands
iii) DNA synthesis
iv) Heating
Which is the correct order?
(Mentioned from starting to ending the reaction)
a.
i)-ii)-iii)-iv)
b.
ii)-i)-iii)-iv)
c.
iv)-iii)-ii)-i)
d.
iv)-ii)-i)-iii)
Posted under Polymerase Chain Reaction Genetic Engineering
Interact with the Community - Share Your Thoughts
Uncertain About the Answer? Seek Clarification Here.
Understand the Explanation? Include it Here.
Q. These are steps taken in carrying out the PCR reaction: i) Attaching of primers by cooling ii) Denaturation of strands iii) DNA synthesis iv) Heating Which is the correct...
Similar Questions
Explore Relevant Multiple Choice Questions (MCQs)
Q. Which of the following is not a condition for PCR?
View solution
Q. Primers and polymerases are added again during the reaction because they get consumed as the reaction proceeds.
View solution
Q. All the molecules generated during PCR will not be full length. Some will also be of intermediate length. Which of the statements is correct?
View solution
Q. Which of the following activity is not present in Taq polymerase?
View solution
Q. Choose the correct statement.
View solution
Q. What is the half life cycle for Taq polymerase?
View solution
Q. Taq polymerase incorporates which residue at 3’ end?
View solution
Q. Polymerases are also available from other Thermus species. Which of the following is correct?
View solution
Q. Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases?
View solution
Q. Thermococcus litoralis grows at a temperature upto 98 degrees.
View solution
Q. Primers are generally ____________
View solution
Q. Which end of the primer should be matched properly in order to carry out the amplification?
View solution
Q. Which of the following nucleotides should be there at 3’ end?
View solution
Q. Melting temperature is given by ____________
View solution
Q. Both the primers, the start primer and the end primer should have a nearly same melting temperature.
View solution
Q. Why internal secondary structures are not preferred for primers?
View solution
Q. Which of the following is favoured for primer design?
View solution
Q. What will happen if the amino acid sequence is used directly for primer designing?
View solution
Q. In the case of uncertainty, if more than one nucleotide is included at a position it is called ____________
View solution
Q. What is the property of inosine?
View solution
Recommended Subjects
Are you eager to expand your knowledge beyond Genetic Engineering? We've handpicked a range of related categories that you might find intriguing.
Click on the categories below to discover a wealth of MCQs and enrich your understanding of various subjects. Happy exploring!
