Q. Primers and polymerases are added again during the reaction because they get consumed as the reaction proceeds.

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Q. All the molecules generated during PCR will not be full length. Some will also be of intermediate length. Which of the statements is correct?

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Q. Which of the following activity is not present in Taq polymerase?

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Q. Choose the correct statement.

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Q. What is the half life cycle for Taq polymerase?

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Q. Taq polymerase incorporates which residue at 3’ end?

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Q. Polymerases are also available from other Thermus species. Which of the following is correct?

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Q. Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases?

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Q. Thermococcus litoralis grows at a temperature upto 98 degrees.

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Q. Primers are generally ____________

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Q. Which end of the primer should be matched properly in order to carry out the amplification?

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Q. Which of the following nucleotides should be there at 3’ end?

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Q. Melting temperature is given by ____________

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Q. Both the primers, the start primer and the end primer should have a nearly same melting temperature.

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Q. Why internal secondary structures are not preferred for primers?

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Q. Which of the following is favoured for primer design?

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Q. What will happen if the amino acid sequence is used directly for primer designing?

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Q. In the case of uncertainty, if more than one nucleotide is included at a position it is called ____________

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Q. What is the property of inosine?

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Q. Which of the following is useful in applications of PCR?

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