Q. Both the primers, the start primer and the end primer should have a nearly same melting temperature.

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Q. Why internal secondary structures are not preferred for primers?

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Q. Which of the following is favoured for primer design?

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Q. What will happen if the amino acid sequence is used directly for primer designing?

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Q. In the case of uncertainty, if more than one nucleotide is included at a position it is called ____________

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Q. What is the property of inosine?

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Q. Which of the following is useful in applications of PCR?

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Q. What is the correct statement with respect to ddNTPs?

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Q. Cycle sequencing is the DNA sequencing where very less amounts of template are utilised for carrying out the sequencing.

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Q. Sickle cell anaemia is a genetic disorder. Which of the following doesn’t holds true for it?

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Q. PCR products can be analysed in many ways. Which of the following is not possible?

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Q. Which of the statements don’t hold true for the forensics and the amplification carried out?

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Q. The genetic relatedness between organisms can be identified by studying the band patterns when different PCR products are analysed electrophoreically. This method is called as ____________

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Q. PCR is useful in population genetics because at times it can be used to study genetics of bacteria that can’t be cultured axenically.

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Q. PCR amplification can be used for which type of samples?

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Q. What is the problem associated with historical DNA samples?

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Q. Which of the statement holds for long-range PCR and in its relation?

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Q. Which of the following conditions don’t contribute to wrong annealing to primer?

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Q. How can the specificity of primer annealing be increased?

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Q. There are basically two types of contamination, laboratory and external. If a PCR product is found to be contaminated by bacteria. It comes under laboratory contamination.

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