Q. What is the problem associated with historical DNA samples?

View solution

Q. Which of the statement holds for long-range PCR and in its relation?

View solution

Q. Which of the following conditions don’t contribute to wrong annealing to primer?

View solution

Q. How can the specificity of primer annealing be increased?

View solution

Q. There are basically two types of contamination, laboratory and external. If a PCR product is found to be contaminated by bacteria. It comes under laboratory contamination.

View solution

Q. Which can be used as a precaution in order to minimize contamination?

View solution

Q. During amplification, there are chances of having a product of a mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it?

View solution

Q. If the template DNA belongs to several individual rather than single one, this type of heterogeneity is known as ____________

View solution

Q. Heterogeneity can also arise if DNA is damaged before amplification. Which of the following doesn’t cause DNA damage?

View solution

Q. If cytosine is deaminated, which of the base is formed?

View solution

Q. Which of the statement is correct for misincorporation?

View solution

Q. Errors can be introduced because of many reasons such as polymerase error or because of heterogeneity. Which of the statement holds true?

View solution

Q. Which of the statement is incorrect for jumping PCR?

View solution

Q. What are the possibilities which can occur until the temperature has reached for primer annealing?

View solution

Q. Hot-start PCR is a modification of PCR. Which of the following is not corresponding to it?

View solution

Q. An alternative to adding polymerase at later stage is __________

View solution

Q. The primer annealing temperature is often very low from the maximum temperature. This low temperature leads to some mismatches.

View solution

Q. Touch-down PCR is another modification. Its characteristics include:

View solution

Q. If two successive PCR are carried out, it is called as __________

View solution

Q. If two successive PCRs are carried out, in which PCR there are chances of having a non-specific product?

View solution