Question

During amplification, there are chances of having a product of a mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it?

a.

Direct sequencing can’t be used in the case if the template DNA is heterozygous at the locus

b.

Direct sequencing can be used if the template DNA is heterozygous at the locus

c.

If cloning is done before sequencing, then it is detected via using only a single clone for sequencing

d.

In the case several recombinants are used, it can’t go undetected

Answer: (b).Direct sequencing can be used if the template DNA is heterozygous at the locus

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Q. During amplification, there are chances of having a product of a mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to...

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