Question
a.
The restriction site is induced into the vector and the topisomerase enzyme is induced into the PCR primers
b.
The topoismerase I is used for cutting both the strands
c.
The induction of topoisomerase enzyme is done into the vector in the case it is very small in size
d.
The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector
Posted under Polymerase Chain Reaction Genetic Engineering
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Q. Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning?
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