Question
a.
Firstly, restriction enzyme digestion is done of those sites whose sequence is not known
b.
Then the self-ligation of molecules is allowed
c.
Now the molecules are cleaved where the known sequence is
d.
Again, the molecules are linearized and the known sequence is in the middle
Posted under Polymerase Chain Reaction Genetic Engineering
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Q. Which one of the following is not done if amplification of the non flanking region is carried out?
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