Q. Which type of PCR allows the use of permeabilized tissue?

View solution

Q. Reverse transciptase PCR is also carried out at times. Which of the statement is true?

View solution

Q. Which one of the following is not done if amplification of the non flanking region is carried out?

View solution

Q. Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers, it is called as __________

View solution

Q. Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning?

View solution

Q. Which of the following statement is incorrect regarding the cloning of PCR products?

View solution

Q. How can PCR product be cloned into a vector?

View solution

Q. The process of inserting an amplified PCR product in a vector for cloning is known as __________

View solution

Q. If two successive PCRs are carried out, in which PCR there are chances of having a non-specific product?

View solution

Q. If two successive PCR are carried out, it is called as __________

View solution

Q. Touch-down PCR is another modification. Its characteristics include:

View solution

Q. The primer annealing temperature is often very low from the maximum temperature. This low temperature leads to some mismatches.

View solution

Q. An alternative to adding polymerase at later stage is __________

View solution

Q. Hot-start PCR is a modification of PCR. Which of the following is not corresponding to it?

View solution

Q. What are the possibilities which can occur until the temperature has reached for primer annealing?

View solution

Q. Which of the statement is incorrect for jumping PCR?

View solution

Q. Errors can be introduced because of many reasons such as polymerase error or because of heterogeneity. Which of the statement holds true?

View solution

Q. Which of the statement is correct for misincorporation?

View solution

Q. If cytosine is deaminated, which of the base is formed?

View solution

Q. Heterogeneity can also arise if DNA is damaged before amplification. Which of the following doesn’t cause DNA damage?

View solution