Question

Which of the following statement is incorrect regarding the cloning of PCR products?

a.

In cloning via restriction enzymes, restriction sites are induced before amplification is carried out

b.

The restriction sites are induced in the primers before annealing

c.

The intermediate molecules are having restriction sites at both ends

d.

The amplified molecules can be cut at both the ends by appropriate enzymes

Posted under Genetic Engineering

Answer: (c).The intermediate molecules are having restriction sites at both ends

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Q. Which of the following statement is incorrect regarding the cloning of PCR products?

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