Question

In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this methodology?

a.

It allows detection of all double stranded molecules

b.

The reporter and quencher are the molecules present on the same probe

c.

The quencher is having a fluorescent group

d.

Fluorescence is observed only when both the groups are present in proximity to each other

Posted under Genetic Engineering

Answer: (b).The reporter and quencher are the molecules present on the same probe

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Q. In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this methodology?

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