Question

Hot-start PCR is a modification of PCR. Which of the following is not corresponding to it?

a.

The basis is that extension is not started until the first cycle reaches its maximum temperature

b.

The polymerase is added after the first cycle has reached its maximum temperature or melting temperature

c.

It is satisfactory for small number of samples

d.

It leads to generation of non specific products

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Answer: (d).It leads to generation of non specific products

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Q. Hot-start PCR is a modification of PCR. Which of the following is not corresponding to it?

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