Question
a.
End-point PCR is favourable over real time PCR
b.
In real time PCR, quantification is done as the reaction is going on
c.
If the product measurement is done after the completion then the measurement is done by target sequence and no other factor affects it
d.
If the primers are available in limited amount, then the product obtained is proportional to the target sequence
Posted under Genetic Engineering
Interact with the Community - Share Your Thoughts
Uncertain About the Answer? Seek Clarification Here.
Understand the Explanation? Include it Here.
Q. Choose the correct statement with regard to quantitative PCR?
Similar Questions
Explore Relevant Multiple Choice Questions (MCQs)
Q. By using two oligonucleotides, we can measure the amount of quantity of DNA by measuring the amount of fluorescence. The first probe absorbs light and the second emits it at a particular wavelength.
View solution
Q. In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this methodology?
View solution
Q. Which of the statement hold true for Quantitative PCR?
View solution
Q. How many approaches are there for measuring the quantity of PCR products?
View solution
Q. Which type of PCR allows the use of permeabilized tissue?
View solution
Q. Reverse transciptase PCR is also carried out at times. Which of the statement is true?
View solution
Q. Which one of the following is not done if amplification of the non flanking region is carried out?
View solution
Q. Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers, it is called as __________
View solution
Q. Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning?
View solution
Q. Which of the following statement is incorrect regarding the cloning of PCR products?
View solution
Q. How can PCR product be cloned into a vector?
View solution
Q. The process of inserting an amplified PCR product in a vector for cloning is known as __________
View solution
Q. If two successive PCRs are carried out, in which PCR there are chances of having a non-specific product?
View solution
Q. If two successive PCR are carried out, it is called as __________
View solution
Q. Touch-down PCR is another modification. Its characteristics include:
View solution
Q. The primer annealing temperature is often very low from the maximum temperature. This low temperature leads to some mismatches.
View solution
Q. An alternative to adding polymerase at later stage is __________
View solution
Q. Hot-start PCR is a modification of PCR. Which of the following is not corresponding to it?
View solution
Q. What are the possibilities which can occur until the temperature has reached for primer annealing?
View solution
Q. Which of the statement is incorrect for jumping PCR?
View solution
Recommended Subjects
Are you eager to expand your knowledge beyond Genetic Engineering? We've handpicked a range of related categories that you might find intriguing.
Click on the categories below to discover a wealth of MCQs and enrich your understanding of various subjects. Happy exploring!
