Question

Why internal secondary structures are not preferred for primers?

a.

Internal structures are very bulky and thus elongation is not preferred

b.

Because of it, primer may fold back on itself and won’t be available for template

c.

Internal secondary structures require more amount of template

d.

If internal structures are present, no proof reading would be observed

Answer: (b).Because of it, primer may fold back on itself and won’t be available for template

Interact with the Community - Share Your Thoughts

Uncertain About the Answer? Seek Clarification Here.

Understand the Explanation? Include it Here.

Q. Why internal secondary structures are not preferred for primers?

Similar Questions

Explore Relevant Multiple Choice Questions (MCQs)

Q. Which of the following is favoured for primer design?

Q. What will happen if the amino acid sequence is used directly for primer designing?

Q. In the case of uncertainty, if more than one nucleotide is included at a position it is called ____________

Q. What is the property of inosine?

Q. Which of the following is useful in applications of PCR?

Q. What is the correct statement with respect to ddNTPs?

Q. Cycle sequencing is the DNA sequencing where very less amounts of template are utilised for carrying out the sequencing.

Q. Sickle cell anaemia is a genetic disorder. Which of the following doesn’t holds true for it?

Q. PCR products can be analysed in many ways. Which of the following is not possible?

Q. Which of the statements don’t hold true for the forensics and the amplification carried out?

Q. The genetic relatedness between organisms can be identified by studying the band patterns when different PCR products are analysed electrophoreically. This method is called as ____________

Q. PCR is useful in population genetics because at times it can be used to study genetics of bacteria that can’t be cultured axenically.

Q. PCR amplification can be used for which type of samples?

Q. What is the problem associated with historical DNA samples?

Q. Which of the statement holds for long-range PCR and in its relation?

Q. Which of the following conditions don’t contribute to wrong annealing to primer?

Q. How can the specificity of primer annealing be increased?

Q. There are basically two types of contamination, laboratory and external. If a PCR product is found to be contaminated by bacteria. It comes under laboratory contamination.

Q. Which can be used as a precaution in order to minimize contamination?

Q. During amplification, there are chances of having a product of a mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it?

Recommended Subjects

Are you eager to expand your knowledge beyond Genetic Engineering? We've handpicked a range of related categories that you might find intriguing.

Click on the categories below to discover a wealth of MCQs and enrich your understanding of various subjects. Happy exploring!