Q. In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme.

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Q. If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?

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Q. If a functional gene is disrupted while disrupting a restriction site _______ is created.

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Q. Additional restriction sites can be introduced near the existing restriction site by mutagenesis.

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Q. Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site.

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Q. Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.

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Q. When a series of deleted regions are replaced by some other DNA fragment of equal length, then it is known as:

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Q. Bisulphite ions are used to deaminate ________ residues in _______ DNA.

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Q. For mutagenesis without PCR, which of the following can be used as a template?

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Q. An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA.

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Q. If the template is double stranded, they need to be separated before annealing of oligonucleotide.

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Q. Which of the following statement is incorrect for the synthesis of the second strand?

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Q. Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

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Q. How many sites can be mutated at a time?

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Q. Several different mutations can be induced at one site.

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Q. If mixed oligonucleotides are used, it is regarded as ______________

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Q. It is easier to subclone a restriction fragment if it belongs to?

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Q. A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

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Q. Choose the correct statement.

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Q. PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

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