Q. Several different mutations can be induced at one site.

View solution

Q. If mixed oligonucleotides are used, it is regarded as ______________

View solution

Q. It is easier to subclone a restriction fragment if it belongs to?

View solution

Q. A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

View solution

Q. Choose the correct statement.

View solution

Q. PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

View solution

Q. Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.

View solution

Q. If PCR is used to introduce random mutations rather than specific mutations, it is called as ___________

View solution

Q. For the selection of the molecules having mutated sequence, which of the statement is true?

View solution

Q. Choose the incorrect statement for the methodology of selection of molecules with mutant sequences.

View solution

Q. Once second strand synthesis is carried out, it is introduced into the host. Host is having which mutation?

View solution

Q. Replication by first strand leads to the formation of mutated molecules and functional antibiotic resistant gene.

View solution

Q. In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?

View solution

Q. What is the function of ung gene?

View solution

Q. DpnI cuts ________ strands.

View solution

Q. The minor change in amino acid sequence can lead to _______ effect on three dimensional structure and there _____ in the primary sequence.

View solution

Q. It is often useful to inactivate endogenous genes in an organism. It might be helpful in finding out _________ role of the wild type gene.

View solution

Q. The inactivation of endogenous genes may also be helpful in directing the expression of the mutated gene in the absence of background expression of wild type gene.

View solution

Q. How can mutant strains be produced?

View solution

Q. The principle of gene disruption is ________ to replace the endogenous chromosomal copy of a gene with __________

View solution