Q. Additional restriction sites can be introduced near the existing restriction site by mutagenesis.

View solution

Q. Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site.

View solution

Q. Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.

View solution

Q. When a series of deleted regions are replaced by some other DNA fragment of equal length, then it is known as:

View solution

Q. Bisulphite ions are used to deaminate ________ residues in _______ DNA.

View solution

Q. For mutagenesis without PCR, which of the following can be used as a template?

View solution

Q. An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA.

View solution

Q. If the template is double stranded, they need to be separated before annealing of oligonucleotide.

View solution

Q. Which of the following statement is incorrect for the synthesis of the second strand?

View solution

Q. Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

View solution

Q. How many sites can be mutated at a time?

View solution

Q. Several different mutations can be induced at one site.

View solution

Q. If mixed oligonucleotides are used, it is regarded as ______________

View solution

Q. It is easier to subclone a restriction fragment if it belongs to?

View solution

Q. A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

View solution

Q. Choose the correct statement.

View solution

Q. PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

View solution

Q. Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.

View solution

Q. If PCR is used to introduce random mutations rather than specific mutations, it is called as ___________

View solution

Q. For the selection of the molecules having mutated sequence, which of the statement is true?

View solution