Q. Natural transformation can be relieved by the use of protoplasts, in the presence of osmotic buffer and polyethylene glycol. What are protoplasts?

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Q. Transfer of plasmids from one cell to another can also be done conjugation. The plasmid to be transferred known as ______ is often unable to do so by itself and thus it relies on other plasmids known as ______

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Q. The transfer of plasmid from one bacterial cell to another when cargo and conjugal plasmids are used, it is usually is carried out by _____ mating.

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Q. One of the commonly used markers is the URA3 gene. It encodes for ______ biosynthesis.

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Q. CAN1 gene encodes a permease that causes uptake of _________ analogue.

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Q. Loss of SUP4 abolishes canavanine uptake, it is arginine analogue.

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Q. Phosphoribosyl amino-imidazole carboxylase is a component of _______ biosynthesis pathway.

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Q. Cells that have lost SUP4 gene acquire _____ pigment and are visibly distinguishable from others.

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Q. Cells mutant in _____ gene can be selected by using 5-fluoro orotic acid, which is turned into ____ products by wild type protein.

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Q. The replicating plasmid vectors in yeast can be divided broadly into how many categories?

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Q. Yeast centromeric plasmid (YCp50) contains ampicillin and tetracycline resistant gene.

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Q. CEN4 in YCp50 is an example of _______

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Q. Yeast episomal plasmids have the following feature?

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Q. Cis-acting REP 3 sequence is present in Yeast episomal plasmids. Choose the statement which holds true for it?

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Q. When YAC is used to clone DNA. What is the size of the DNA that can be cloned?

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Q. TEL is the ______ sequence derived from the ends of ribosomal RNA-encoding molecules from the macromolecules of the protozoan Tetrahyena.

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Q. Choose the incorrect statement for YAC vectors.

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Q. If YAC DNA is digested with BamHI and ECoRI, how many fragments are generated?

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Q. Transformed cells in the case of YAC can be selected by the presence of both URA3 and TRP1 genes.

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Q. GAL1 is a controllable promoter and is obtained from genes for galactose metabolism. It is known for encoding ______

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