Q. Cells that have lost SUP4 gene acquire _____ pigment and are visibly distinguishable from others.

View solution

Q. Cells mutant in _____ gene can be selected by using 5-fluoro orotic acid, which is turned into ____ products by wild type protein.

View solution

Q. The replicating plasmid vectors in yeast can be divided broadly into how many categories?

View solution

Q. Yeast centromeric plasmid (YCp50) contains ampicillin and tetracycline resistant gene.

View solution

Q. CEN4 in YCp50 is an example of _______

View solution

Q. Yeast episomal plasmids have the following feature?

View solution

Q. Cis-acting REP 3 sequence is present in Yeast episomal plasmids. Choose the statement which holds true for it?

View solution

Q. When YAC is used to clone DNA. What is the size of the DNA that can be cloned?

View solution

Q. TEL is the ______ sequence derived from the ends of ribosomal RNA-encoding molecules from the macromolecules of the protozoan Tetrahyena.

View solution

Q. Choose the incorrect statement for YAC vectors.

View solution

Q. If YAC DNA is digested with BamHI and ECoRI, how many fragments are generated?

View solution

Q. Transformed cells in the case of YAC can be selected by the presence of both URA3 and TRP1 genes.

View solution

Q. GAL1 is a controllable promoter and is obtained from genes for galactose metabolism. It is known for encoding ______

View solution

Q. Transcription of GAL1 and GAL10 is suppressed in the presence of _______

View solution

Q. How many deletion variations of GAL1 promoter are there?

View solution

Q. Promoters such as GAL1 can be used to direct synthesis of either unmodified or fusion proteins.

View solution

Q. Which of the following are advantageous to be used for expression of eukaryotic cells?

View solution

Q. Secretion of overexpressed proteins by fusion to a secretion signal can be useful because it helps in bringing about correct _______ and _____ protein folding.

View solution

Q. The enzyme used for breaking down of sucrose into glucose and fructose is called as _______________

View solution

Q. For introduction of exogenous DNA into S.cerevisiae ______ is added along with polyethylene glycol and heat shock treatment.

View solution