Q. Mutant strains of Saccharomyces cervevisiae in which endogenous DNA are deleted are called as _________

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Q. COX3 gene is a selectable marker. Choose the correct statement with respect to it.

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Q. The ARG8m gene which produces an enzyme for arginine biosynthesis is located in _______ and is of ______ origin.

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Q. Barstar is _________

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Q. Caenorhabditis elegans is a model organism of great importance in biological systems. It is a/an _________

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Q. DNA can be injected into Caenorhabditis elegans by biolistic transformation. The injected DNA forms arrays of extrochromosomal copies which are stable in nature.

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Q. Autographa californica nuclear polyhedrois virus (AcNPV) is commonly used bacilovirus for infecting cultured cells. The virus is ________ and _______

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Q. DNA replication of the AcNPV takes 6 hours after infection. After how many hours of infection new virus particles are produced by budding?

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Q. The virus particles are held together by polyhedron and p10 protein.

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Q. The polyhedrin and p10 protein are produced in ____________ amount and other genes are expressed in _________ amount.

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Q. Expression of proteins using nuclear polyhedrosis viruses is advantageous because it gives _______ protein yields and post-translational modifications are _________

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Q. The size of the viral genome is large and thus poses a problem. This problem can be resolved by using ____________

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Q. The gene of interest is inserted into transfer vector and then further into viral genome leading to the formation of modified virus. How can plaques from modified virus differentiated from plaques from wild-type virus?

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Q. If the DNA is linearized, what is the effect on the recombination frequency?

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Q. The recombinant virus can be propagated in E.coli and this is referred to as bacmid.

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Q. For the generation of recombinant baculovirus, recombinants can be selected by ____________

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Q. Transgenic Drosophila can be created by microinjection of DNA into the embryos. These embryos are at which stage of development?

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Q. Syncytium is a layer of ______ that have not been separated into individual cells.

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Q. In Drosophila, only nuclei cells give rise to germline cells.

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Q. ______ integration systems are used for the transfer of DNA in Drosophila and it is composed of ______

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