Q. ______ integration systems are used for the transfer of DNA in Drosophila and it is composed of ______

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Q. Only the _____ part of Drosophila is transgenic and the rest is not. This is known as ______

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Q. rosy gene is used as a selectable marker for transformation in Drosophila, it produces an enzyme required for the synthesis of ____________

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Q. Wild type flies have crimson red eyes whereas rosy mutant have brick-red eyes.

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Q. Wild-type Drosophila flies are _____ to ethanol supplied in food.

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Q. Cis-acting sites should be present in the vector for ________

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Q. The host should ____ P elements, these elements lead to _______

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Q. Where do P elements integrate into the genome?

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Q. Transient gene silencing can be carried out by microinjecting ______

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Q. Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following?

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Q. Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation.

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Q. Cells deficient in thymidine kinase (TK) are also killed in HAT medium.

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Q. Hygromycin is used as a selectable marker in mammalian cultured cells. It is used for ____________

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Q. Puromycin is a protein synthesis inhibitor. It is conferred by _______ gene.

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Q. Resistance to bleomycin (zeocin) is used as a selectable marker for mammalian cultured cells and its function is ____________

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Q. Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as a selectable marker. This enzyme is involved in the synthesis of _____ carbon units and is required for _____ biosynthesis.

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Q. Histidinol dehydrogenase allows synthesis of histidine from exogenous histanol.

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Q. Many mammalian cells contain Thymidine Kinase, the mammalian enzyme uses the analogue _____ than does the viral enzyme.

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Q. A wide range of host cell lines are available and commonly used human cell lines are obtained from ___________

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Q. DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______

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