Q. Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following?

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Q. Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation.

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Q. Cells deficient in thymidine kinase (TK) are also killed in HAT medium.

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Q. Hygromycin is used as a selectable marker in mammalian cultured cells. It is used for ____________

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Q. Puromycin is a protein synthesis inhibitor. It is conferred by _______ gene.

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Q. Resistance to bleomycin (zeocin) is used as a selectable marker for mammalian cultured cells and its function is ____________

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Q. Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as a selectable marker. This enzyme is involved in the synthesis of _____ carbon units and is required for _____ biosynthesis.

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Q. Histidinol dehydrogenase allows synthesis of histidine from exogenous histanol.

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Q. Many mammalian cells contain Thymidine Kinase, the mammalian enzyme uses the analogue _____ than does the viral enzyme.

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Q. A wide range of host cell lines are available and commonly used human cell lines are obtained from ___________

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Q. DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______

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Q. Cells whose contents have been removed and replaced, by swelling and shrinking in solutions of suitable osmotic strength are called as ____________

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Q. In ___________ cells, virus replication doesn’t take place and viral DNA ___________

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Q. SV40 is a virus and it produces how many transcripts?

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Q. Splicing of the transcripts is necessary for efficient expression.

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Q. T protein is required for replication. It is a product of ____________

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Q. Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _____________ and can accept at least ____________ of foreign DNA.

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Q. Vaccinia virus produces its own ______ which is functional in _____ of infected cells where the virus also replicates.

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Q. It is beneficial to use retrovirus for genetic manipulation of cells in the culture.

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Q. LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.

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